Lan Report - Dna Biochem - 2332 Words

ASSIGNMENT 1: PROPERTIES OF DNA/RNA Introduction: The polymerase chain reaction is an innovative technology, which amplifies a single piece of DNA across several orders of magnitude. The end result is the creation of thousands to millions of copies of a particular DNA sequence. PCR is closely patterned after the natural principle of DNA replication. It is a three-step process, referred to as a cycle, that is repeated a specified number of times. One PCR cycle consists of the following steps: * Denaturation * Annealing * Extension This process takes place in a thermal cycles, usually between 30 and 40 cycles. In the initial step, heat (usually hotter than 90 degrees Celsius) separates double-stranded DNA into†¦show more content†¦Figure 1: Electrophoresis Gel Pattern for Sex Determination A photograph of an agrose gel pattern. DNA obtained by scrapings from epithelial lining of subjects mouth was synthesized with ologioucucleotide primers specific to either the X chromosome or Y chromosome, thus allowing for the determination of the sex of the subject by assessing the migration appearance of precipitated DNA after agrose gel electrophoresis. Molecular weight marker were loaded in the middle lane (Lane 6) which consisted of small DNA ladder containing fragmented of DNA sized 1000, 900, 800, 700, 600, 500, 400, 300, 200 and 100bp which acted as a control. Lane 1: Mouth Scrapping DNA Lane 2: Intact Genomic E.Coli DNA Lane 3: Restriction Enzyme Lane 4: pUC 19 DNA Lane 5: PUC 19 digested Hinf I Lane 6: pUC 19 digested Hinf I (Ethanol Precipitated) Lane 7: Large DNA ladder size standard Lane 8: E.Coli RNA/DNA – not treated Lane 9: Not Loaded Lane 10: E.Coli RNA/DNA – Finger RNAse treated Lane 11: E.Coli RNA/DNA – Commercial ribonuclease treated Lane 12: E.Coli RNA/DNA – NaOH treated Lane 13: Yeast RNA Lane 1: Mouth Scrapping DNA Lane 2: Intact Genomic E.Coli DNA Lane 3: Restriction Enzyme Lane 4: pUC 19 DNA Lane 5: PUC 19 digested Hinf I Lane 6: pUC 19 digested Hinf I (Ethanol Precipitated) Lane 7: Large DNA ladder size standard Lane 8: E.Coli RNA/DNA – not treated Lane 9: Not Loaded Lane 10: E.Coli RNA/DNA – Finger RNAse